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===Diagnostic approaches===
 
===Diagnostic approaches===
 
* Toxigenic Culture (TC) and/or Cell Cytotoxicity Assay (CCA)
 
* Toxigenic Culture (TC) and/or Cell Cytotoxicity Assay (CCA)
** Anaerobic culture for 24-48 hrs followed by colony selection and culture with
+
** Anaerobic culture for 24-48 hrs followed by colony selection and culture with human cells to test for cell cytotoxicity which takes an additional 24-48 hours.
human cells to test for cell cytotoxicity which takes an additional 24-48 hours.
   
* Gold standard
 
* Gold standard
 
** Time consuming and difficult, up to 5 days
 
** Time consuming and difficult, up to 5 days

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